解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::It has previously been shown that the genome of RF virus, a variant of the human papovavirus, BK, consists of two DNA species, one (R1a) with a deletion corresponding to the early and the other (R2) with a deletion corresponding to the late region of BKV (A. Pater, M.M. Pater, and G. di Mayorca (1980). J. Virol. 36, 4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90509-3
更新日期:1983-12-01 00:00:00
abstract::The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90510-x
更新日期:1983-12-01 00:00:00
abstract::In this paper we present a technique developed to physically locate the HSV-1 gene(s) which restore neurovirulence to a non-neurovirulent HSV intertypic recombinant described in the preceding report. In brief, tissue culture cells are co-transfected with unit length RE6 DNA and restriction endonuclease fragmented HSV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90544-5
更新日期:1983-11-01 00:00:00
abstract::Nineteen independent temperature-sensitive (ts) mutants were isolated from SA11 following mutagenesis with proflavin or 5-azacytidine. Fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. Five of the ts mutants defined five recombination groups (F, G, H, I, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90100-9
更新日期:1983-10-30 00:00:00
abstract::The synthesis and processing of the nucleocapsid protein (pp60) of the JHM strain of murine coronaviruses were examined. Pulse-chase experiments showed that pp60 was synthesized initially as a protein of approximately 57,000 in molecular weight (p57). Immunoprecipitation using mouse anti-JHMV antiserum indicated that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90106-x
更新日期:1983-10-30 00:00:00
abstract::Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90139-3
更新日期:1983-10-15 00:00:00
abstract::The genome of the single-stranded DNA phage f1 contains an intergenic region (IG), 508-nucleotides long, that does not code for any known protein. By use of a system of chimeric plasmids haboring different f1 fragments, we had previously shown that this region contains, in addition to the f1 'functional origin' of DNA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90136-8
更新日期:1983-10-15 00:00:00
abstract::The amino acid sequence of BMV (brome mosaic virus) coat protein is presented and compared with the sequence derived from RNA structure. Apart from removal of the N-terminal methionine residue and acetylation of the following serine residue, no post-translational modifications occur. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90193-9
更新日期:1983-09-01 00:00:00
abstract::Plasmids containing the E1 region of Ad12 DNA can transform certain rodent cells into oncogenic cells. To study the role of the E1a subregion in the process of oncogenic transformation, Ad12 region E1 mutants carrying deletions in the E1a region were constructed. Deletion mutants pR7 and pR8 affect only the 13 S mRNA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90178-2
更新日期:1983-09-01 00:00:00
abstract::A mutant (dp 201) of human adenovirus type 2 has been isolated that has the left 3% (1110 bp) of the genome duplicated at the right terminus. The structure of the mutant genome was studied by restriction endonuclease analysis and by direct DNA sequence analysis. The duplication has resulted in a deletion of 10 bp of t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90177-0
更新日期:1983-09-01 00:00:00
abstract::Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90409-9
更新日期:1983-08-01 00:00:00
abstract::Genetic variation in dengue 2 isolates from various geographic areas was examined by oligonucleotide fingerprinting of the 40 S genome RNA. Oligonucleotide maps of geographically isolated and epidemiologically unrelated viruses were very distinct. Direct comparison of the oligonucleotide map of the dengue 2 prototype ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90255-6
更新日期:1983-07-30 00:00:00
abstract::A quantitative analysis of Epstein-Barr virus (EBV)-induced early antigen (EA) and viral capsid antigen (VCA) syntheses was carried out in Raji cells superinfected with purified, concentrated P3HR-1 EBV. When the cells were exposed to the virus and assessed by immunofluorescence and immunoprecipitation, EA induction o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90276-3
更新日期:1983-07-30 00:00:00
abstract::Mouse LTK- cells (H-2k) were transfected with a series of recombinant plasmids consisting of the herpes simplex virus type 1 thymidine kinase (TK) gene linked to fragments of SV40 DNA coding for portions of SV40 T antigen in pBR322, and TK+ transformants (LTK+) were selected in HAT medium. The TK+ transformants were a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90259-3
更新日期:1983-07-30 00:00:00
abstract::Inbred mice bearing the FV-1n marker when inoculated at birth with an ecotropic murine leukemia virus (WM 1504-E) obtained from wild mice develop a progressive central nervous system (CNS) disease manifested by hindlimb paralysis and incoordination that begins by 6 to 7 weeks of age. Studies of infected SWR/J mice at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90326-4
更新日期:1983-07-15 00:00:00
abstract::The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modifie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90150-2
更新日期:1983-06-01 00:00:00
abstract::Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90382-3
更新日期:1983-05-01 00:00:00
abstract::A number of factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus SA11 have been examined. (1) Statistical analyses of recombination frequency (RF) indicated that (a) the variability noted in RF was not related to variations in experimental conditions and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90374-4
更新日期:1983-05-01 00:00:00
abstract::In order to determine the location and biological significance of the acid-induced conformational change in influenza virus hemagglutinin (HA) reported by Skehel et al., monoclonal antibodies were prepared to the molecule before and after treatment at pH 5.0. These antibodies together with monoclonal antibodies to the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)80015-4
更新日期:1983-04-30 00:00:00
abstract::The relationship between the cell cycle and the presence of the nuclear T antigen in simian virus 40-transformed rat cells independently derived from FR 3T3 cells has been investigated using a fluorescence-activated cell sorter and a combination of stainings for cellular DNA (4',6-diamidine-2-phenylindole dihydrochlor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)80028-2
更新日期:1983-04-30 00:00:00
abstract::The extent of viral DNA methylation in non-virus-producing benign and malignant tumors induced by the Shope (rabbit) papilloma virus was investigated with the two isoschizomeric restriction endonucleases MspI and HpaII. The overall extent of methylation is variable in papillomas and uniformly higher in carcinomas. In ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)80007-5
更新日期:1983-04-30 00:00:00
abstract::Incubation of membrane vesicles from normal and Rous sarcoma virus-transformed chick embryo fibroblasts (CEF) with [gamma-32P]ATP resulted in the phosphorylation of a large number of proteins. The major differences observed between the membrane vesicles of untransformed and transformed cells were: (1) a 5- to 10-fold ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90462-2
更新日期:1983-04-15 00:00:00
abstract::The regions of the AcMNPV genome represented as cytoplasmic transcripts early after infection of Spodoptera frugiperda cells prior to and encompassing the initiation of DNA replication were mapped. In vivo(32)P pulse-labeled cytoplasmic RNA from infected cells at various times postinfection was used to probe Southern ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90491-9
更新日期:1983-04-15 00:00:00
abstract::Two revertants of ts110 Moloney murine sarcoma virus (MuSV) with wild-type MuSV phenotype were examined for the presence of mos gene products, ts110 MuSV has a temperature-sensitive defect in a function required to maintain the transformed phenotype. The nonproducer 6m2 cell clone transformed by ts110 produces an 85,0...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90483-x
更新日期:1983-04-15 00:00:00
abstract::Very fast sedimenting DNA (VFS DNA) of T4 phage, which is formed by infection with a mutant in gene 49, was examined by electron microscopy after mild treatment with DNase I. It showed Y-shaped, branched strands in addition to linear strands. Each branch contained a single-stranded interruption about 60 nucleotides lo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90470-1
更新日期:1983-04-15 00:00:00
abstract::The Gardner (GA) and Snyder-Theilen (ST) isolates of feline sarcoma virus (FeSV) represent genetic recombinants between feline leukemia virus (FeLV) and transformation-specific sequences (v-fes gene) of cat cellular origin. A related transforming gene (v-fps), common to the Fujinami, PRC II, and UR 1 strains of avian ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90219-2
更新日期:1983-03-01 00:00:00
abstract::Purification of the putative cowpea mosaic virus (CPMV) RNA replicase previously started with an RNA-dependent RNA polymerase activity which had been solubilized from a crude membrane fraction of CPMV-infected cowpea leaves by extraction with a Mg2+-deficient buffer. This led to the identification of a host-encoded, 1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90071-5
更新日期:1983-02-01 00:00:00
abstract::Experiments were undertaken to determine if the sera of avian sarcoma virus-infected 15I5 X 7(2) chickens exhibit antibody reactivity for species of endogenous retroviral envelope glycoprotein expressed in 15I5 X 7(2) fibroblasts. Two viruses were used for infection of the 15I5 X 7(2) chickens, Pr-A and cl. 85; the en...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90348-3
更新日期:1983-01-30 00:00:00
abstract::Rat 3Y1 cells were infected by AKR virus through microinjection of molecularly cloned proviral DNA. Based on a strong immunofluorescence using anti-p30 as an antiserum a cell clone (RESA-2) was selected that had a high expression of viral antigens. Subsequent restriction analysis of its DNA revealed that the RESA-2 cl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90361-6
更新日期:1983-01-30 00:00:00
abstract::Avian myelocytomatosis virus MC29 inoculated into the wing web of 1-day-old Brown Leghorn chickens causes a high incidence of tumors, predominantly endotheliomas that are apparently induced directly by the action of the viral myc gene. Mutants of MC29, in which portions of the v-myc gene have been deleted and which ha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90300-8
更新日期:1983-01-15 00:00:00
abstract::Mouse hybridomas which produce monoclonal antibodies to the Autographa californica nuclear polyhedrosis virus (AcNPV) have been prepared and the protein to which each is directed has been determined. The antibody produced by clones 3F3 and 5E9 specifically bound polyhedron protein of MW 32,000 when used in immunoadsor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90241-0
更新日期:1982-10-30 00:00:00
abstract::Cellular organelles of cauliflower mosaic virus-infected turnip plants were isolated, incubated with 32P-labeled deoxyribonucleoside triphosphates and assayed for their ability to synthesize virus-specific DNA. Only nuclei were found to synthesize a labeled DNA product. This DNA migrated during agarose gel electrophor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90124-6
更新日期:1982-08-01 00:00:00
abstract::The bottom component B1a of TYMV with a buoyant density of 1.42-1.43 g/ml, is converted to a more dense particle B2a following CsCI density gradient centrifugation. This conversion is complete after a prior incubation of the virus in the CsCl solution at elevated temperatures. It is shown that the formation of B28 is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90041-1
更新日期:1982-07-30 00:00:00
abstract::The amino acid sequence of the coat protein of wild-type cowpea chlorotic mottle virus has been nearly completely determined by direct methods. The sites of amino acid replacements were identified in the coat proteins of an oxidation-sensitive mutant, a temperature-sensitive mutant, and a salt-stable mutant. The repla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90108-8
更新日期:1982-06-01 00:00:00
abstract::The results of a previous study suggested that the poly(A) sequence in barley stripe mosaic virus (BSMV) RNA is intercalated between a 3'-terminal tyrosine-accepting structure and the 5'-terminal coding part of the BSMV genome. Here we show that poly(A)+ and poly(A)- fractions of BSMV RNA can be cleaved into two fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90064-2
更新日期:1982-05-01 00:00:00
abstract::Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Transla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90326-9
更新日期:1982-04-15 00:00:00
abstract::A mutant (Tbts7) of alfalfa mosaic virus, the coat protein of which is unable to activate the viral genome (the RNA species 1, 2, and 3, which need some coat protein for infectivity) at 30 degrees , can be rescued at this temperature by adding to the inoculum wild-type RNA 3 (the genome part that contains the coat pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90528-1
更新日期:1982-02-01 00:00:00
abstract::The nucleic acid of carnation cryptic virus has been identified as double-stranded RNA on the basis of (i) susceptibility to RNase in 0.1 x SSC (SSC = 0.15 M NaCl-0.015 M sodium citrate buffer, pH 7.0) and resistance to RNase in 2 x SSC and to DNase, (ii) reaction with an antiserum containing antibodies to dsRNA, and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90124-0
更新日期:1981-12-01 00:00:00
abstract::The allele Pp of gene ref(2)P of Drosophila melanogaster is known to inhibit the multiplication of certain strains of Sigma virus. From a viral strain for which the allele PP of ref(2)P is nonpermissive, host adapted (haP) mutants were selected after mutagenesis in vivo. Mutagenized virus was extracted from infected f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90227-0
更新日期:1981-10-31 00:00:00
abstract::Tobacco mosaic virus (TMV) RNA was encapsulated in large unilamellar vesicles (LUV) of phosphatidylserine and was introduced into protoplasts from Vinca rosea suspension cultures. Infection could be effected by brief incubation of protoplasts with the LUV containing TMV-RNA in the presence of either polyethylene glyco...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90203-8
更新日期:1981-09-01 00:00:00